Preparation of 10% emamectin benzoate soluble granule for trunk injection and its control efficacy on pine wilt disease
Received:March 11, 2019    Download the full
DOI：10.16801/j.issn.1008-7303.2019.0061
Key Words:trunk injection  emamectin benzoate  soluble granule  pine wilt disease  conduction  control effect
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Author NameAffiliationE-mail
ZHANG Xinwei Local and National Joint Engineering Laboratory of Biopesticide High-efficient Preparation Technology, Zhejiang A & F University, Lin'an 311300, Zhejiang Province, China  
HAN Qunqi Local and National Joint Engineering Laboratory of Biopesticide High-efficient Preparation Technology, Zhejiang A & F University, Lin'an 311300, Zhejiang Province, China  
WANG Tianna Lin'an Municipal Bureau of Agricultural and Rural Affairs, Lin'an 311300, Zhejiang Province, China  
WU Liping Forestry Bureau of Jiande, Jiande 311600, Zhejiang Province, China  
CHEN Anliang Local and National Joint Engineering Laboratory of Biopesticide High-efficient Preparation Technology, Zhejiang A & F University, Lin'an 311300, Zhejiang Province, China anlchen@126.com 
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Abstract:
      In order to prevent and control pine wilt disease, 10% emamectin benzoate soluble granule was prepared for trunk injection. Its control efficacy against pine wood nematode disease was evaluated after trunk injection. Sodium benzoate, lactose monohydrate, soluble starch and CMC were tested as the water-soluble carrier. The formulation of the water-soluble carrier was optimized using the uniform design of experiments with mixtures and partial least squares regression. The emamectin benzoate (oil phase) was dissolved in n-butanol and emulsifier OP-10, and mixed with water-soluble carrier with different mixing time. And then the optimal formulation and mixing time was obtained by tracking and detecting of the indicators. The optimum formulation of emamectin benzoate 10% soluble granule was composed of emamectin benzoate 14.3% (pure 10.0%), OP-10 5.7%, n-butanol 12%, sodium benzoate 39.0%, lactose monohydrate 20.5%, water-solubility starch 3.0% and CMC 4.5%. After 10 min mechanical kneading, the appearance of the granule was the cylinder in milk white. And all the indicators were up to the standard. The field experiments showed that the granule could completely dissolve in water and effectively conduct in P. massoniana after the trunk injection. In the pine wood nematode disease area in Donghu Village, 110 pines were injected. The mortality of P. massoniana was 0 after 1 year. And the mortality of P. massoniana in the control group was 6.1%. In the Xian' gong Mountain, 230 pines were injected. The mortality of P. massoniana was 1.7%, and the mortality of P. massoniana in the control group was 10.8%. The soluble granule can be used during the whole pine resin secretion period. And it features simple and safe production procedur, no packaging pollution and significant control effect.
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