Sensitivity analysis of Colletotrichum spp. from wolfberry to four sterol demethylation inhibitors in Gansu Province
Received:November 27, 2018    Download the full
DOI:10.16801/j.issn.1008-7303.2019.0074
Key Words:Colletotrichum spp.  Colletotrichum gloeosporioides species complex  Colletotrichum acutatum species complex  sterol demethylation inhibitors(DMIs)  difenoconazole  tebuconazole  propiconazole  flusilazole  sensitivity
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Author NameAffiliationE-mail
ZHANG Haiying Institute of Plant Protection, Gansu Academy of Agricultural Science, Lanzhou 730070, China
Scientific Observing and Experimental Station of Crop Pests in Tianshui, Ministry of Agriculture and Rural Affairs, P. R. China, Gangu 741299, Gansu Province, China 
 
LIU Yonggang Institute of Plant Protection, Gansu Academy of Agricultural Science, Lanzhou 730070, China
Scientific Observing and Experimental Station of Crop Pests in Tianshui, Ministry of Agriculture and Rural Affairs, P. R. China, Gangu 741299, Gansu Province, China 
 
LI Jianjun Institute of Plant Protection, Gansu Academy of Agricultural Science, Lanzhou 730070, China
Scientific Observing and Experimental Station of Crop Pests in Tianshui, Ministry of Agriculture and Rural Affairs, P. R. China, Gangu 741299, Gansu Province, China 
 
ZHANG Xinrui Institute of Plant Protection, Gansu Academy of Agricultural Science, Lanzhou 730070, China
Scientific Observing and Experimental Station of Crop Pests in Tianshui, Ministry of Agriculture and Rural Affairs, P. R. China, Gangu 741299, Gansu Province, China 
zxr@gsagr.ac.cn 
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Abstract:
      The objective of this work is to evaluate the sensitivity of Colletotrichum spp. isolated from wolfberry in Gansu Province to sterol demethylation inhibitors (DMIs). The petri plate was adopted to test the sensitivities of 102 isolates of Colletotrichum spp. from wolfberry at three different regions in Jingyuan and three different regions in Jingtai in Gansu province to 4 DMIs fungicides including difenoconazole, tebuconazole, propiconazole and flusilazole. The sensitivity differentiation of C. gloeosporioides species complex and C. acutatum species complex collected indifferent years and from different regions to four fungicides was analyzed respectively. The results indicated that the overall sensitivities of 46 C. gloeosporioides species complex to difenoconazole, tebuconazole, propiconazole and flusilazole were high. EC50 value ranged from 0.28 to 1.20 μg/mL, 0.11 to 2.98 μg/mL, 0.32 to 2.84 μg/mL and 0.35 to 3.85 μg/mL, respectively. However, the sensitivities of 56 tested C. acutatum species complex isolates to four DMIs fungicides were different and some strains were suspected to have decreased sensitivity. The average EC50 values of the least sensitive strains to difenoconazole, tebuconazole, propiconazole and flusilazole are 1.63,3.8,6.21 and 4.74 μg/mL, respectively. The sensitivities of C. gloeosporioides species complex and C. acutatum species complex collected in different years to those 4 DMIs fungicides showed significant difference and the collected strains in 2017 showed relatively lower sensitivities. The average EC50 values of C. gloeosporioides species complex to four DMIs fungicides respectively were (0.84 ±0.03), (1.23 ±0.13), (1.19 ±0.09) and (1.69 ±0.17) μg/mL, respectively. The average EC50 values of C. acutatum species complex to four DMIs fungicides respectively were (1.06 ±0.03), (2.25 ±0.15), (2.43 ±0.20) and (2.85 ±0.19)μg/mL, respectively. The sensitivities of Colletotrichum spp. isolates collected from different areas to those 4 fungicides were different. The strains of C. gloeosporioides species complex collected from Wuhe Jingyuan county had the lowest sensitivity. The average EC50 values were (0.79 ±0.12), (1.28 ±0.87), (1.39 ±1.05) and (1.74 ±1.04) μg/mL, respectively. The strains with the highest sensitivity to difenoconazole and propiconazole were collected from Caowotan Jingtai county, and the average EC50 values were (0.28 ±0.10) and (0.46 ±0.10) μg/mL, respectively. The strains with the highest sensitivity to tebuconazole and flusilazole were collected from Sitan Jingtai county, and the average EC50 values were (0.42 ±0.16) and (0.65 ±0.09) μg/mL, respectively. There was no significant difference among the sensitivities of C. acutatum species complex from different areas to those 4 fungicides. The results provided the theoretical basis for the rational use of fungicides to control wolfberry anthracnose in Gansu Province and delaying resistance development.
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