Isolation and identification of chemical constituents of the aerial parts of Astragalus complanatus and their antibacterial activities
Received:March 05, 2019    Download the full
DOI:10.16801/j.issn.1008-7303.2019.0038
Key Words:Astragalus complanatus  chemical constituents  isolation and identification  antibacterial activity
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Author NameAffiliationE-mail
XUE Lijuan College of Plant Protection, Northwest A & F University, Yangling 712100, Shaanxi Province, China  
JI Zhiqin College of Plant Protection, Northwest A & F University, Yangling 712100, Shaanxi Province, China
Shaanxi Province Key Laboratory Research & Development on Botanical Pesticides, Northwest A & F University, Yangling 712100, Shaanxi Province, China 
jizhiqin@nwsuaf.edu.cn 
WEI Shaopeng College of Plant Protection, Northwest A & F University, Yangling 712100, Shaanxi Province, China
Shaanxi Key Laboratory of Phytochemistry, Baoji 721000, Shaanxi Province, China 
 
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Abstract:
      For the comprehensive utilization of medicinal plant Astragalus complanatus, the chemical constituents of its the non-meidcinal parts and their antibacterial activities were investigated preliminarily. Seven compounds were isolated by using silica gel and LH-20 gel chromatography, and their antibacterial activities against 8 species of bacteria were evaulated. After spectrometric analysis, the obtained compounds were identified as β-sitosterol (1), β-sitostenone (2), lawsaritol A (3), 8-epidioxy-5α,8α-ergosta-6,22E-dien-3β-ol (4), 3',7-dihydroxy-2',4'-dimethoxyisoflavan (5), D-1-O-methyl inositol (6) and complanatuside (7). All of the above compounds were isolated from the aerial parts of A. complanatus for the first time. Compound 5 showed inhibition effect on Bacillus cereus, Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Erwinia carotovora, Pseudomonas syringae pv. actinidiae and Ralstonia solanacearum, and the MIC values of compound 5 against three species of plant pathogenic bacteria, including P. syringae, R. solanacearum and E. carotovora, were 37.5, 18.8 and 75 μg/mL, respectively. To our best knowledge, this is the first report about the inhibition effect of 5 on plant pathogenic bacteria.
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