Rapid detection of 102 pesticides residues in Angelica sinensis using gas chromatography-tandem mass spectrometry
Received:December 09, 2018    Download the full
DOI：10.16801/j.issn.1008-7303.2019.0039
Key Words:QuEChERS  gas chromatography-tandem mass spectrometry (GC-MS/MS)  dynamic multi response monitoring (dMRM)  Angelica sinensis  rapid screening  pesticide residues
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Author NameAffiliationE-mail
YAN Jun Lanzhou Institutes for Food and Drug Control, Lanzhou 730050, China  
ZHAO Bo Lanzhou Institutes for Food and Drug Control, Lanzhou 730050, China 123859811@qq.com 
ZHANG Wen Lanzhou Institutes for Food and Drug Control, Lanzhou 730050, China  
WA Xiaoxia Basic Medical College, Gansu University of Chinese Medicine, Lanzhou 730030, China  
WANG Xiaoqiao Lanzhou Institutes for Food and Drug Control, Lanzhou 730050, China  
CHEN Ting Lanzhou Institutes for Food and Drug Control, Lanzhou 730050, China  
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Abstract:
      Established a rapid determination method for 102 kinds of pesticide residues in angelica sinensis of genuine medicinal materials in Gansu. Optimized the QuEChERS pretreatment method, used pattern detection with gas chromatography-tandem mass spectrometry (GC-MS/MS), dynamic multi response monitoring (dMRM), and matrix matching external standard method, studied the influence of extraction agent, extraction system, composition and dosage of purification system on sample extraction and purification in the pre-treatment process of angelica pesticide residues, as well as the influence of matrix effect on the detection results. Optimized and used acetonitrile, 1.0 g sodium chloride, 4.0 g anhydrous magnesium sulfate, 1.0 g sodium citrate, 0.5 g disodium citrate for extraction, and 800 mg anhydrous magnesium sulfate, 150 mg PSA, 150 mg C18 for purification. The influence of matrix effect on qualitative and quantitative on target compounds in angelica significantly reduced by the correction with matrix matching external standard method. The methodological validation indicators was well that 102 kinds of pesticide residues show a good linear relationship within the range of 0.02 mg/kg to 0.64 mg/kg, and the R2 was greater than 0.99. Method detection limit is from 0.002 5 mg/kg to 0.025 0 mg/kg. Method quantitative limit is from 0.005 0 mg/kg to 0.050 0 mg/kg. The average recovery rate was from 52% to 128%, and the RSD was from 1.0 % to 10%. The method was rapid, simple, time-consuming and has good sensitivity, accuracy and precision, provided a fast, efficient and reliable analytical method for improve the level of pesticide safety in angelica sinensis and reduce health risks.
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