Residues and dissipation dynamics of spirotetramat and its metabolites in pear and soil
Received:October 02, 2018    Download the full
DOI:10.16801/j.issn.1008-7303.2019.0044
Key Words:ultra performance liquid chromatography-tandem mass spectrometry  spirotetramat  metabolite  pear  soil  residue  dissipation dynamics
Fund Project:
Author NameAffiliationE-mail
QIAN Xun Research Center of Quality and Safety of Agro-products, Hebei Academy of Agriculture and Forestry Sciences, Laboratory of Quality and Safety Risk Assessment for Agro-products(Shijiazhuang), Ministry of Agriculture and Rural Affairs, Shijiazhuang 050051, China  
ZHENG Zhenshan Research Center of Quality and Safety of Agro-products, Hebei Academy of Agriculture and Forestry Sciences, Laboratory of Quality and Safety Risk Assessment for Agro-products(Shijiazhuang), Ministry of Agriculture and Rural Affairs, Shijiazhuang 050051, China  
CHEN Yongda Research Center of Quality and Safety of Agro-products, Hebei Academy of Agriculture and Forestry Sciences, Laboratory of Quality and Safety Risk Assessment for Agro-products(Shijiazhuang), Ministry of Agriculture and Rural Affairs, Shijiazhuang 050051, China  
ZHANG Shaojun Research Center of Quality and Safety of Agro-products, Hebei Academy of Agriculture and Forestry Sciences, Laboratory of Quality and Safety Risk Assessment for Agro-products(Shijiazhuang), Ministry of Agriculture and Rural Affairs, Shijiazhuang 050051, China zhangshj601@163.com 
GUAN Junfeng Research Center of Quality and Safety of Agro-products, Hebei Academy of Agriculture and Forestry Sciences, Laboratory of Quality and Safety Risk Assessment for Agro-products(Shijiazhuang), Ministry of Agriculture and Rural Affairs, Shijiazhuang 050051, China  
FAN Lixin Research Center of Quality and Safety of Agro-products, Hebei Academy of Agriculture and Forestry Sciences, Laboratory of Quality and Safety Risk Assessment for Agro-products(Shijiazhuang), Ministry of Agriculture and Rural Affairs, Shijiazhuang 050051, China  
ZHAO Xudong Research Center of Quality and Safety of Agro-products, Hebei Academy of Agriculture and Forestry Sciences, Laboratory of Quality and Safety Risk Assessment for Agro-products(Shijiazhuang), Ministry of Agriculture and Rural Affairs, Shijiazhuang 050051, China  
QIAN Mengyuan Research Center of Quality and Safety of Agro-products, Hebei Academy of Agriculture and Forestry Sciences, Laboratory of Quality and Safety Risk Assessment for Agro-products(Shijiazhuang), Ministry of Agriculture and Rural Affairs, Shijiazhuang 050051, China  
Hits: 183
Download times: 122
Abstract:
      A method for the simultaneous determination of spirotetramat and its metabolites such as spirotetramat-enol-glucoside(S-glu), spirotetramat-keto-hydroxy(S-keto), spirotetramat-enol (S-enol) and spirotetramat-mono-hydroxy(S-mono) in pear and soil was developed. The residual dissipation dynamics of spirotetramat in pears was determined. The residues in samples were extracted with acetonitrile(containing 1% acetic acid) and cleaned up by dispersivemethod with N-propylethylenediamine (PSA) and anhydrous magnesium sulfate as cleaning agents. Then the samples were detected by ultra performance liquid chromatography-tandem massspectrometry under selective reaction monitoring mode, and quantified by external standard method. The results showed good linearity (R2 ≥ 0.999) between mass concentration and peak area of mass spectra of each compound when the concentrations of spirotetramat ranged from 0.005 to 0.10 mg/L; S-glu from 0.005 to 0.5 mg/L; S-keto, S-enol and S-mono from 0.000 5 to 0.5 mg/L, respectively. When the fortified levels ranged from 0.005 to 0.7 mg/kg, the average fortified recoveries in pear were in the range of 84% to 109% and the reletive standard deviations (RSD) were 1.2%-3.3%. The average fortified recoveries in soil were in the range of 86% to 102% and the RSD were 1.1%-3.6%. The limits of quantification(LOQ) were 5 μg/kg. The method is rapid, sensitive and reproducible. Therefore, It is suitable for the rapid detection and identification of spirotetramat and its metabolite residues in pear and soil. According to the recommended dosage, the total residues of spirotetramat and its metabolites were 0.023-0.056 mg/kg in the fruit and 0-0.015 mg/kg in the soil when the pear was ripe and harvested. The half-life of spirotetramat in pear fruit was 12.4 d, and the half-life in soil was 7.1 d. Therefore, it is safe to use spirotetramat for the pest control of pear tree.
View Fulltext  View/Add Comment  Download reader