In vitro bioactivities of four fungicides including carbendazim against Botrytis cinerea in tobacco
Received:November 08, 2018    Download the full
DOI:10.16801/j.issn.1008-7303.2019.0033
Key Words:tobacco grey mould  Botrytis cinerea  carbendazim  pyrimethanil  iprodione  propiconazole  sensitivity  control efficacy
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Author NameAffiliationE-mail
ZHOU Hao College of Life Sciences, Yangtze University, Jingzhou 434025, Hubei Province, China
Guizhou Academy of Tobacco Sciences, Guiyang 550081, China 
 
LI Licui College of Life Sciences, Yangtze University, Jingzhou 434025, Hubei Province, China
Guizhou Academy of Tobacco Sciences, Guiyang 550081, China 
 
FAN Jie College of Life Sciences, Yangtze University, Jingzhou 434025, Hubei Province, China
Guizhou Academy of Tobacco Sciences, Guiyang 550081, China 
 
YU Zhihe College of Life Sciences, Yangtze University, Jingzhou 434025, Hubei Province, China  
WANG Hancheng Guizhou Academy of Tobacco Sciences, Guiyang 550081, China xiaobaiyang126@hotmail.com 
CHEN Xingjiang Guizhou Academy of Tobacco Sciences, Guiyang 550081, China  
MA Jun Guizhou Provincial Qianxinan Tobacco Company, Xingyi 562400, Guizhou Province, China  
LIU Jinhua Guizhou Provincial Qianxinan Tobacco Company, Xingyi 562400, Guizhou Province, China majun161@163.com 
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Abstract:
      The in vitro bioactivities of four fungicides (carbendazim, iprodione, pyrimethanil, propiconazole) against Botrytis cinerea from tobacco were investigated using mycelial growth rate method and spore germination method. Their protective and curative activities against grey mould on detached leaves were investigated. The results showed that the four fungicides had different inhibitory activities. The fungicide with the highest toxic potential against the mycelial growth of B. cinerea was carbendazim with average EC50 value of 0.06 mg/L, followed by propiconazole, pyrimethanil and iprodione, with average EC50 values of 0.36, 0.53 and 0.60 mg/L, respectively. The EC50 values of fungicides against spore germination were as followings:iprodione (2.05 mg/L), propiconazole (2.21 mg/L), pyrimethanil (10.56 mg/L) and carbendazim (131.23 mg/L). The most effective fungicide with the best protective and curative activities was iprodione and carbendazim. At the concentration of 200 mg/L, protective control efficacies were both 100% and the curative efficacies were 98.3% and 91.8%, respectively. This study provided scientific basis for guiding the control of tobacco grey mould.
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