Fermentation condition optimization of isopropylstilbene and its inhibition effect against Botrytis cinerea
Received:September 27, 2018    Download the full
DOI：10.16801/j.issn.1008-7303.2019.0026
Key Words:Photohabdus temperata  isopropylstilbene  cultural condition optimization  Botrytis cinerea  antifungal activity  gray mold  control effect
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Author NameAffiliationE-mail
WANG Baojuan Colledge of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China  
CAI Nan Colledge of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China  
LI Xinghai Colledge of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China  
YU Zhiguo Colledge of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China
Engineering & Technological Research Center of Biopesticide, Shenyang 110866, China 		yu_zhiguo2012@163.com 
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Abstract:
      Isopropylstilbene (3, 5-dihydroxy-4-isopropyl stilbene) is a stilbene compound produced by Photohabdus temperata, which has various biological activities. In order to investigate the potential application of isopropylstilbene in the field of agricultural antibiotics, this compound was isolated from the fermentation broth of Photohabdus temperata SN35 by solid phase extraction and and purified by column chromatography. Its structure was confirmed by 1H NMR and the purity was determined by high performance liquid chromatography. The shake flask fermentation conditions of the Photohabdus temperata SN35 were optimized by the Plackett-Burman test and the response surface method. The in vitro and in vivo antifungal activities of the compound against Botrytis cinerea were studied. The results showed that the isolated compound was isopropylstilbene with the purity of 93%. The yield of isopropylstilbene was 2.22 times higher than that before the optimization. The in vitro test results showed that the EC50 value of isopropylstilbene against the mycelial growth of B. cinerea was (9.17 ±0.17) μg/mL, which was lower than that of the control, chlorothalonil[EC50 value (0.90 ±0.08) μg/mL]. And at 10 μg/mL, the inhibition rate against spore germination was 63.01%. The results of pot experiments showed that the control effect of isopropylstilbene on cucumber gray mould was 92.19% at 200 μg/mL, which was almost the same with the control effect of chlorothalonil (97.63%). The control effect on tomato gray mold was 72.12%, which slightly lower than that of chlorothalonil (81.61%). This study laid a theoretical foundation for the industrial production by optimizing the fermentation conditions of isopropylstilbene. The compound has a good control effect against gray mold which will be useful for the research and development of natural fungicides.
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