Effects of trans-cinnamaldehyde against Villosiclava virens on cell ultrastructure and activities of three respiratory enzymes
Received:January 30, 2019    Download the full
DOI：10.16801/j.issn.1008-7303.2019.0031
Key Words:trans-cinnamaldehyde  Villosiclava virens  cell ulstrastructure  nicotinamide adenine dinucleotide-malic dehydrogenase  succinate dehydrogenase  adenosine triphosphatase
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Author NameAffiliationE-mail
ZHENG Jingge Institute of Plant Protection, Chinese Academy of Agricultural Sciences/Key Laboratory of Control of Biological Hazard Factors (Plant Origin) for Agri-product Quality and Safety, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Beijing 100193, China  
GUO Zhixin Institute of Plant Protection, Chinese Academy of Agricultural Sciences/Key Laboratory of Control of Biological Hazard Factors (Plant Origin) for Agri-product Quality and Safety, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Beijing 100193, China  
LIU Tingting Institute of Plant Protection, Chinese Academy of Agricultural Sciences/Key Laboratory of Control of Biological Hazard Factors (Plant Origin) for Agri-product Quality and Safety, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Beijing 100193, China  
MAO Liangang Institute of Plant Protection, Chinese Academy of Agricultural Sciences/Key Laboratory of Control of Biological Hazard Factors (Plant Origin) for Agri-product Quality and Safety, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Beijing 100193, China  
ZHANG Yanning Institute of Plant Protection, Chinese Academy of Agricultural Sciences/Key Laboratory of Control of Biological Hazard Factors (Plant Origin) for Agri-product Quality and Safety, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Beijing 100193, China  
ZHANG Lan Institute of Plant Protection, Chinese Academy of Agricultural Sciences/Key Laboratory of Control of Biological Hazard Factors (Plant Origin) for Agri-product Quality and Safety, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Beijing 100193, China lanzhang@ippcaas.cn 
JIANG Hongyun Institute of Plant Protection, Chinese Academy of Agricultural Sciences/Key Laboratory of Control of Biological Hazard Factors (Plant Origin) for Agri-product Quality and Safety, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Beijing 100193, China ptnpc@vip.163.com 
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Abstract:
      The antifungal mechanism of trans-cinnamaldehyde (TC) against Villosiclava virens was investigated. The alteration of cell ultrastructure was observed using transmission electron microscope. The integrity of cell wall and activities of nicotinamide adenine dinucleotide-malic dehydrogenase (NAD-MDHase), succinate dehydrogenase (SDHase) and adenosine triphosphatase (ATPase) of V. virens were determined after treated with TC at the mass concentration of 2, 4, 8, 15 and 30 μg/mL. The results showed that the contents of lipidosome increased significantly and the structure of mitochondrion was indiscernible after treatment with TC at the mass concentration of 4 μg/mL. With the mass concentration of TC increasing to 30 μg/mL, the integrity of cell wall in V. virens was destroyed and the relative inhibition of NAD-MDHase and SDHase were reduced by 44.3% and 76.7%, respectively. Differently, the activities of Na+-K+-ATPase, Ca2+-Mg2+-ATPase, Ca2+-ATPase and Mg2+-ATPase fluctuated as U-curve with the concentration increase of TC. When the mass concentration of TC was 4 μg/mL, the highest inhibitive activity of TC on ATPase was the achieved among all the tested concentrations and the relative inhibition of TC on ATPase was higher than 78.4%. These results suggested that the cell wall and mitochondrion were the possible action targets for TC to inhibit the mycelial growth.
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