甘肃省枸杞炭疽病菌对4种甾醇脱甲基抑制剂的敏感性
投稿时间:2018-11-27      点此下载全文 HTML
引用本文:张海英,刘永刚,李建军,等.甘肃省枸杞炭疽病菌对4种甾醇脱甲基抑制剂的敏感性[J].农药学学报,2019,21(4):424-430.
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张海英 甘肃省农业科学院 植物保护研究所, 兰州 730070
农业农村部天水作物有害生物科学观测实验站, 甘肃 甘谷 741299 
 
刘永刚 甘肃省农业科学院 植物保护研究所, 兰州 730070
农业农村部天水作物有害生物科学观测实验站, 甘肃 甘谷 741299 
 
李建军 甘肃省农业科学院 植物保护研究所, 兰州 730070
农业农村部天水作物有害生物科学观测实验站, 甘肃 甘谷 741299 
 
张新瑞 甘肃省农业科学院 植物保护研究所, 兰州 730070
农业农村部天水作物有害生物科学观测实验站, 甘肃 甘谷 741299 
zxr@gsagr.ac.cn 
基金项目:甘肃省农业科学院科研条件建设及成果转化项目(2017GAAS24);甘肃省农业科学院农业科技创新专项(2017GAAS23);甘肃省农业科学院中青年基金项目(2015GAAS37)
中文摘要:为明确甘肃省枸杞炭疽病菌对甾醇脱甲基抑制剂类药剂(DMIs)的敏感性,采用菌丝生长速率法测定了采自甘肃省靖远县3个地区及景泰县3个地区共102株枸杞炭疽病菌对苯醚甲环唑、戊唑醇、丙环唑及氟硅唑的敏感性,分别就不同年份、不同地区间胶孢炭疽复合种和尖孢炭疽复合种对4 种 DMIs 杀菌剂的敏感性差异进行了分析。结果表明:供试46 株枸杞胶孢炭疽复合种整体上对苯醚甲环唑、戊唑醇、丙环唑和氟硅唑仍表现为敏感,EC50值分别在0.28~1.20、0.11~2.98、0.32~2.84和0.35~3.85 μg/mL之间;而56株尖孢炭疽复合种对4种药剂的敏感性则出现了不同程度分化,部分菌株疑似已出现敏感性下降现象,其中,对苯醚甲环唑、戊唑醇、丙环唑和氟硅唑敏感性最低的菌株EC50值分别为1.63、3.80、6.21和4.74 μg/mL。不同年份间采集的枸杞胶孢炭疽复合种和尖孢炭疽复合种对4种杀菌剂的敏感性均存在显著差异,2017年采集的菌株敏感性相对更低,4种杀菌剂对胶孢炭疽复合种的平均EC50值分别为(0.84 ±0.03)、(1.23 ±0.13)、(1.19 ±0.09)和(1.69 ±0.17)μg/mL,对尖孢炭疽复合种的平均EC50值分别为(1.06 ±0.03)、(2.25 ±0.15)、(2.43 ±0.20)和(2.85 ±0.19)μg/mL。不同地区枸杞炭疽病菌对4种杀菌剂的敏感性表现不同,其中靖远县五合镇的胶孢炭疽复合种对4种杀菌剂敏感性最低,平均EC50值分别为(0.79 ±0.12)、(1.28 ±0.87)、(1.39 ±1.05)和(1.74 ±1.04)μg/mL,景泰县草窝滩镇的胶孢炭疽复合种对苯醚甲环唑和丙环唑敏感性最高,平均EC50值为(0.28 ±0.10)和(0.46 ±0.10)μg/mL,对戊唑醇和氟硅唑敏感性最高的胶孢炭疽复合种来自景泰县寺滩乡,平均EC50值为(0.42 ±0.16)和(0.65 ±0.09)μg/mL;不同地区间采集的尖孢炭疽复合种对4种杀菌剂的敏感性则不存在显著差异。研究结果可为甘肃省枸杞炭疽病防治中杀菌剂的合理使用及延缓抗药性发展提供依据。
中文关键词:枸杞炭疽病菌  胶孢炭疽复合种  尖孢炭疽复合种  甾醇脱甲基抑制剂  苯醚甲环唑  戊唑醇  丙环唑  氟硅唑  敏感性
 
Sensitivity analysis of Colletotrichum spp. from wolfberry to four sterol demethylation inhibitors in Gansu Province
Abstract:The objective of this work is to evaluate the sensitivity of Colletotrichum spp. isolated from wolfberry in Gansu Province to sterol demethylation inhibitors (DMIs). The petri plate was adopted to test the sensitivities of 102 isolates of Colletotrichum spp. from wolfberry at three different regions in Jingyuan and three different regions in Jingtai in Gansu province to 4 DMIs fungicides including difenoconazole, tebuconazole, propiconazole and flusilazole. The sensitivity differentiation of C. gloeosporioides species complex and C. acutatum species complex collected indifferent years and from different regions to four fungicides was analyzed respectively. The results indicated that the overall sensitivities of 46 C. gloeosporioides species complex to difenoconazole, tebuconazole, propiconazole and flusilazole were high. EC50 value ranged from 0.28 to 1.20 μg/mL, 0.11 to 2.98 μg/mL, 0.32 to 2.84 μg/mL and 0.35 to 3.85 μg/mL, respectively. However, the sensitivities of 56 tested C. acutatum species complex isolates to four DMIs fungicides were different and some strains were suspected to have decreased sensitivity. The average EC50 values of the least sensitive strains to difenoconazole, tebuconazole, propiconazole and flusilazole are 1.63,3.8,6.21 and 4.74 μg/mL, respectively. The sensitivities of C. gloeosporioides species complex and C. acutatum species complex collected in different years to those 4 DMIs fungicides showed significant difference and the collected strains in 2017 showed relatively lower sensitivities. The average EC50 values of C. gloeosporioides species complex to four DMIs fungicides respectively were (0.84 ±0.03), (1.23 ±0.13), (1.19 ±0.09) and (1.69 ±0.17) μg/mL, respectively. The average EC50 values of C. acutatum species complex to four DMIs fungicides respectively were (1.06 ±0.03), (2.25 ±0.15), (2.43 ±0.20) and (2.85 ±0.19)μg/mL, respectively. The sensitivities of Colletotrichum spp. isolates collected from different areas to those 4 fungicides were different. The strains of C. gloeosporioides species complex collected from Wuhe Jingyuan county had the lowest sensitivity. The average EC50 values were (0.79 ±0.12), (1.28 ±0.87), (1.39 ±1.05) and (1.74 ±1.04) μg/mL, respectively. The strains with the highest sensitivity to difenoconazole and propiconazole were collected from Caowotan Jingtai county, and the average EC50 values were (0.28 ±0.10) and (0.46 ±0.10) μg/mL, respectively. The strains with the highest sensitivity to tebuconazole and flusilazole were collected from Sitan Jingtai county, and the average EC50 values were (0.42 ±0.16) and (0.65 ±0.09) μg/mL, respectively. There was no significant difference among the sensitivities of C. acutatum species complex from different areas to those 4 fungicides. The results provided the theoretical basis for the rational use of fungicides to control wolfberry anthracnose in Gansu Province and delaying resistance development.
Key words:Colletotrichum spp.  Colletotrichum gloeosporioides species complex  Colletotrichum acutatum species complex  sterol demethylation inhibitors(DMIs)  difenoconazole  tebuconazole  propiconazole  flusilazole  sensitivity
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