| 扁茎黄芪植株中化学成分的分离鉴定及其抑菌活性 |
|
投稿时间：2019-03-05
点此下载全文
HTML
|
| 引用本文：薛利娟,姬志勤,魏少鹏.扁茎黄芪植株中化学成分的分离鉴定及其抑菌活性[J].农药学学报,2019,21(3):389-394. |
| 摘要点击次数: 2776 |
| 全文下载次数: 830 |
|
| 基金项目:国家重点研发计划资助（2017YFD0201402）；陕西省教育厅重点实验室科研计划项目（访学项目，13JS008） |
|
| 中文摘要:为综合开发利用药用植物扁茎黄芪Astragalus complanatus，对其非药用部位的化学成分及抑菌活性进行了初步研究。采用硅胶柱层析和LH-20凝胶层析技术从扁茎黄芪植株中分离鉴定了7个化合物，并测定了各化合物对8种细菌的抑菌活性。根据波谱分析结果，7个化合物分别被鉴定为β-谷甾醇（1）、β-sitostenone（2）、lawsaritol A（3）、3β-羟基-5α，8α-桥二氧麦角甾-6，22-二烯（4）、3'，7-二羟基-2'，4'-二甲氧基异黄烷（5）、D-1-O-甲基肌醇（6） 和沙苑子苷（7），所有化合物均为首次从扁茎黄芪植株中分离得到。抑菌活性测定结果表明：化合物5对蜡状芽孢杆菌Bacillus cereus、大肠杆菌Escherichia coli、金黄色葡萄球菌Staphylococcus aureus、枯草芽孢杆菌Bacillus subtilis、白菜软腐病菌Erwinia carotovora、猕猴桃溃疡病菌Pseudomonas syringae pv.actinidiae和烟草青枯病菌Ralstonia solanacearum均有不同程度的抑制作用，其中对猕猴桃溃疡病菌、烟草青枯病菌和白菜软腐病菌的最低抑制浓度（MIC）分别为37.5、18.8和75.0 μg/mL。3'，7-二羟基-2'，4'-二甲氧基异黄烷（5） 的农用抑菌活性为首次报道。 |
| 中文关键词:扁茎黄芪 化学成分 分离鉴定 抑菌活性 |
| |
| Isolation and identification of chemical constituents of the aerial parts of Astragalus complanatus and their antibacterial activities |
|
|
| Abstract:For the comprehensive utilization of medicinal plant Astragalus complanatus, the chemical constituents of its the non-meidcinal parts and their antibacterial activities were investigated preliminarily. Seven compounds were isolated by using silica gel and LH-20 gel chromatography, and their antibacterial activities against 8 species of bacteria were evaulated. After spectrometric analysis, the obtained compounds were identified as β-sitosterol (1), β-sitostenone (2), lawsaritol A (3), 8-epidioxy-5α,8α-ergosta-6,22E-dien-3β-ol (4), 3',7-dihydroxy-2',4'-dimethoxyisoflavan (5), D-1-O-methyl inositol (6) and complanatuside (7). All of the above compounds were isolated from the aerial parts of A. complanatus for the first time. Compound 5 showed inhibition effect on Bacillus cereus, Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Erwinia carotovora, Pseudomonas syringae pv. actinidiae and Ralstonia solanacearum, and the MIC values of compound 5 against three species of plant pathogenic bacteria, including P. syringae, R. solanacearum and E. carotovora, were 37.5, 18.8 and 75 μg/mL, respectively. To our best knowledge, this is the first report about the inhibition effect of 5 on plant pathogenic bacteria. |
| Key words:Astragalus complanatus chemical constituents isolation and identification antibacterial activity |
|
查看全文
查看/发表评论
下载PDF阅读器 |
|
|
|
