| 基于高效液相色谱-三重四极杆串联质谱技术测定鲜荔枝果肉中10种有机酸含量 |
|
投稿时间：2018-10-19
点此下载全文
HTML
|
| 引用本文：王思威,刘艳萍,王潇楠,等.基于高效液相色谱-三重四极杆串联质谱技术测定鲜荔枝果肉中10种有机酸含量[J].农药学学报,2019,21(3):359-365. |
| 摘要点击次数: 3035 |
| 全文下载次数: 931 |
|
| 基金项目:国家荔枝龙眼产业技术体系（CARS-32-16）；广东省重点领域研发科技计划项目（2018B020202011）；广东省农业科学院院长基金项目（201929） |
|
| 中文摘要:为明确鲜荔枝果肉中各有机酸的含量及对风味的影响，采用高效液相色谱-三重四极杆串联质谱技术，建立了鲜荔枝果肉中苹果酸、柠檬酸等10种主要有机酸含量的测定方法。样品采用Poroshell色谱柱分离，以甲酸水-乙腈为流动相等度洗脱，在电喷雾离子源负离子（ESI?）及多反应离子监测模式下进行分析，外标法定量。结果显示：在0.1~10 mg/L范围内，10种有机酸进样质量浓度与峰面积间线性关系良好，相关系数（r）大于0.99，方法的检出限（LOD）为0.1~0.8 μg/kg，定量限（LOQ）为0.2~2.5 μg/kg。10种有机酸在鲜荔枝果肉中的平均添加回收率为91%~99%，相对标准偏差（RSD）为1.9%~7.9%。该方法快速、简便、灵敏，可用于鲜荔枝样品中10种有机酸的测定，同时可为其他水果中有机酸的测定提供参考。 |
| 中文关键词:高效液相色谱-串联质谱 荔枝 有机酸 苹果酸 柠檬酸 |
| |
| Determination of ten organic acids content in fresh litchi based on high performance liquid chromatography with triple quadrupole mass spectrometry technique |
|
|
| Abstract:In order to determine the amount of organic acids in litchi and their effects on the flavor of litchi, an effective method was developed for the determination of ten organic aicds (including malic acid, citric acid and other organic aicds) in fresh litchi by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Poroshell chromatographic column was used as the separation column. Formic acid water and acetonitrile was used as the mobile phase. The electrospray ion source and negative ion muptiple reaction monitoring mode were also used. The analytes were quantified by the external standard method, and the pretreatment and mass spectrometry conditions were evaluated. Ten organic acids showed good linearities in the range of 0.1-10 mg/L, and the correlation coefficients (r) were all above 0.99. The limits of detection (LODs) were 0.1-0.8 μg/kg, and the limits of quantification (LOQs) were 0.2-2.5 μg/kg. The average recoveries of ten organic acids in fresh litchi samples were 91%-99% with the relative standard deviations of 1.9%-7.9%. This method is rapid, easy and sensitive. Therefore, it is suitable for the quick determination and analysis of ten organic acids in fresh litchi, and it can also provide reference for the determination of organic acids in other fruits. |
| Key words:high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) Litchi chinensis organic acids malic acid citric acid |
|
查看全文
查看/发表评论
下载PDF阅读器 |
