抑霉唑的毛细管电泳手性拆分及其在线富集
投稿时间：2019-03-21      点此下载全文 HTML
引用本文：虞婕,许杭杰,赵美蓉,等.抑霉唑的毛细管电泳手性拆分及其在线富集[J].农药学学报,2019,21(3):309-314.
摘要点击次数: 3464
全文下载次数: 791
作者单位E-mail
虞婕 浙江工业大学 环境学院, 杭州 310029  
许杭杰 浙江工业大学 环境学院, 杭州 310029  
赵美蓉 浙江工业大学 环境学院, 杭州 310029  
岳思青 浙江工业大学 环境学院, 杭州 310029 yuesiqing@zjut.edu.cn 
基金项目:国家自然科学基金（21677130）；中国博士后科学基金（2018M632503）
中文摘要:采用毛细管电泳法对抑霉唑进行手性拆分，研究了手性选择剂β-环糊精（β-CD）、有机添加剂、NaH2PO4、NH4H2PO4和分离电压对手性拆分的影响；同时，采用堆积法对抑霉唑进行在线富集，研究了进样压力和进样时间对在线富集的影响。结果表明：在分离电压为20 kV、β-CD浓度为5 mmol/L及缓冲体系为2.5% 异丙醇 + 75 mmol/L NaH2PO4 + 5 mmol/L NH4H2PO4时，抑霉唑获得最佳分离度，分离度可达3.0；在分离电压为20 kV、β-CD浓度为5 mmol/L、缓冲体系为75 mmol/L NaH2PO4 + 5 mmol/L NH4H2PO4 + 50 mmol/L H3PO4及进样条件为13.8 kPa×99.9 s时获得最高富集倍数，富集倍数达91~92倍。
中文关键词:抑霉唑  毛细管电泳  手性拆分  堆积法  在线富集
 
Enantioseparation and on-column preconcentrstion of imazalil by capillary electrophoresis
Abstract:The enantioseparation of imazalil was conducted by capillary electrophoresis, and the effects of chiral selector β-cyclodextrin(β-CD), organic additives, NaH2PO4, NH4H2PO4 and separation voltage on the resolution were studied. And the effects of the injection pressure and preconcentration times on the on-column preconcentration of imazalil were studied by the stacking method. The results showed that the best resolution was achieved under the following conditions: separation voltage 20 kV, β-CD 5 mmol/L and a buffer system of 2.5 % isopropanol + 75 mmol/L NaH2PO4 + 5 mmol/L NH4H2PO4 . And the resolution was up to 3.0. The highest enrichment factor was obtained at the separation voltage of 20 kV using 5 mmol/L β-CD, a buffer system of 75 mmol/L NaH2PO4 + 5 mmol/L NH4H2PO4 + 50 mmol/L H3PO4, and the injection conditions of 13.8 kPa×99.9 s. And the enrichment factor was up to 91-92 times.
Key words:imazalil  capillary electrophoresis  enantioseparation  stacking method  on-column preconcentration
查看全文   查看/发表评论   下载PDF阅读器