多菌灵等4种杀菌剂对烟草灰霉病菌的室内生物活性
投稿时间:2018-11-08      点此下载全文 HTML
引用本文:周浩,李丽翠,樊杰,等.多菌灵等4种杀菌剂对烟草灰霉病菌的室内生物活性[J].农药学学报,2019,21(2):238-243.
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作者单位E-mail
周浩 长江大学 生命科学学院, 湖北 荆州 434025
贵州省烟草科学研究院, 贵阳 550081 
 
李丽翠 长江大学 生命科学学院, 湖北 荆州 434025
贵州省烟草科学研究院, 贵阳 550081 
 
樊杰 长江大学 生命科学学院, 湖北 荆州 434025
贵州省烟草科学研究院, 贵阳 550081 
 
余知和 长江大学 生命科学学院, 湖北 荆州 434025  
汪汉成 贵州省烟草科学研究院, 贵阳 550081 xiaobaiyang126@hotmail.com 
陈兴江 贵州省烟草科学研究院, 贵阳 550081  
马骏 贵州省烟草公司 黔西南州公司, 贵州 兴义 562400  
刘锦华 贵州省烟草公司 黔西南州公司, 贵州 兴义 562400 majun161@163.com 
基金项目:国家自然科学基金(31501679);贵州省科技厅优秀青年人才培养计划(黔科合平台人才[2017]5619);贵州省科技支撑计划(黔科合支撑[2018]2356);中国烟草总公司贵州省公司科技项目(201711,201714)
中文摘要:采用菌丝生长速率法和孢子萌发法,分别测定了烟草灰霉病菌对多菌灵、嘧霉胺、异菌脲和丙环唑的敏感性,同时通过离体叶片法评估了这4种杀菌剂对烟草灰霉病的保护和治疗作用。结果表明:4种杀菌剂对烟草灰霉病菌的菌丝生长和孢子萌发均表现出了不同程度的抑制活性,并对灰霉病同时具有保护和治疗作用。其中多菌灵对菌丝生长的抑制活性最强,EC50平均值为0.06 mg/L,其次为丙环唑、嘧霉胺和异菌脲,EC50平均值分别为0.36、0.53和0.60 mg/L;异菌脲和丙环唑对烟草灰霉病菌孢子萌发的抑制活性较强,EC50平均值分别为2.05和2.21 mg/L,其次为嘧霉胺和多菌灵,EC50平均值分别为10.56和131.23 mg/L。异菌脲和多菌灵对灰霉病的保护作用和治疗作用均最强,当药剂质量浓度为200 mg/L时,其对离体叶片的保护和治疗作用防效分别为100%、100%和98.3%、91.8%。研究结果可为烟草灰霉病的科学防治提供依据。
中文关键词:烟草灰霉病  灰葡萄孢  多菌灵  嘧霉胺  异菌脲  丙环唑  敏感性  防治效果
 
In vitro bioactivities of four fungicides including carbendazim against Botrytis cinerea in tobacco
Abstract:The in vitro bioactivities of four fungicides (carbendazim, iprodione, pyrimethanil, propiconazole) against Botrytis cinerea from tobacco were investigated using mycelial growth rate method and spore germination method. Their protective and curative activities against grey mould on detached leaves were investigated. The results showed that the four fungicides had different inhibitory activities. The fungicide with the highest toxic potential against the mycelial growth of B. cinerea was carbendazim with average EC50 value of 0.06 mg/L, followed by propiconazole, pyrimethanil and iprodione, with average EC50 values of 0.36, 0.53 and 0.60 mg/L, respectively. The EC50 values of fungicides against spore germination were as followings:iprodione (2.05 mg/L), propiconazole (2.21 mg/L), pyrimethanil (10.56 mg/L) and carbendazim (131.23 mg/L). The most effective fungicide with the best protective and curative activities was iprodione and carbendazim. At the concentration of 200 mg/L, protective control efficacies were both 100% and the curative efficacies were 98.3% and 91.8%, respectively. This study provided scientific basis for guiding the control of tobacco grey mould.
Key words:tobacco grey mould  Botrytis cinerea  carbendazim  pyrimethanil  iprodione  propiconazole  sensitivity  control efficacy
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