QuEChERS-超高效液相色谱-串联质谱法测定果蔬中螺虫乙酯、丁醚脲及代谢物残留 |
投稿时间:2018-10-28
点此下载全文
HTML
|
引用本文:兰丰,姚杰,周先学,等.QuEChERS-超高效液相色谱-串联质谱法测定果蔬中螺虫乙酯、丁醚脲及代谢物残留[J].农药学学报,2019,21(2):219-226. |
摘要点击次数: 4133 |
全文下载次数: 1153 |
|
基金项目:国家农产品质量安全风险评估项目(GJFP2019003);山东省农业科学院农业科技创新工程:山东省主要农产品质量安全风险评估与控制技术研究(CXGC2016B17) |
|
中文摘要:建立了QuEChERS前处理-超高效液相色谱-串联质谱(UPLC-MS/MS)测定果蔬中螺虫乙酯、丁醚脲及其6种代谢物(螺虫乙酯-烯醇、螺虫乙酯-烯醇-糖苷、螺虫乙酯-单羟基、螺虫乙酯-酮-羟基、丁醚脲-脲和丁醚脲-甲酰胺)残留量的分析方法。样品经破壁机匀浆,V(乙腈):V(甲酸)=10:1混合溶液涡旋提取,定制的QuEChERS净化管净化,岛津Shim-pack XR-ODS色谱柱分离(75 mm×2.0 mm,1.6 μm)。采用电喷雾离子源正离子多反应监测模式进行质谱检测,外标法定量。丁醚脲-脲和丁醚脲-甲酰胺在0.001~0.1 mg/L范围内线性关系良好,其他6种化合物在0.005~0.5 mg/L范围内线性关系良好,R2 ≥ 0.999 3。验证了梨、葡萄、西瓜、芥蓝、芹菜和韭菜6种基质下方法的准确度和精密度,在0.001~0.5 mg/kg添加水平下,8种化合物的平均回收率在68%~94%之间,相对标准偏差在5.4%~15%之间,定量限为0.001~0.005 mg/kg。该方法简便、快速、可靠,适用于同时测定果蔬中上述8种化合物残留。 |
中文关键词:QuEChERS 超高效液相色谱-串联质谱 果蔬 螺虫乙酯 丁醚脲 代谢产物 残留 |
|
Residue of spirotetramat, diafenthiuron and their metabolites in fruits and vegetables by QuEChERS-ultra performance liquid chromatography-tandem mass spectrometry |
|
|
Abstract:A method was developed for the quantitative determination of spirotetramat, diafenthiuron and their metabolite residues (spirotetramat-enol, spirotetramat-enol-glucoside, spirotetramat-mono-hydroxy, spriotetramat-keto-hydroxy, diafenthiuron-urea, diafenthiuron-amide) by QuEChERS-ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The samples were homogenized, extracted with V (acetonitrile):V(formic acid)=10:1, and cleaned-up by custom QuEChERS tubes. The separation was performed on a Shimadu Shim-pack XR-ODS column(75 mm×2.0 mm, 1.6 μm). The compounds were identified by MS/MS in positive electrospray ionization (ESI+) and multiple reaction monitoring (MRM) mode, and quantified by external standard method. The linearities of diafenthiuron-urea and diafenthiuron-amide ranged from 0.001 to 0.1 mg/L. And that of the other six compounds ranged from 0.005 to 0.5 mg/L, with the correlation coeffecients higher than 0.999 3. At the fortified level from 0.001 mg/kg to 0.5 mg/kg in pear, grape, watermelon, kale, celery and leek, the average recoveries of all the eight compounds ranged from 68% to 94% with the relative standard deviations varied from 5.4% to 15%. The limits of quantification (LOQ) of the analytes were 0.001-0.005 mg/kg. The method is simple, rapid, accurate and suitable for the confirmation and quantification of those eight compounds in fruits and vegetables. |
Key words:QuEChERS ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) fruits and vegetables spirotetramat diafenthiuron metabolites residue |
查看全文
查看/发表评论
下载PDF阅读器 |
|
|
|