| 河朔荛花根中抑菌活性成分研究 |
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投稿时间：2018-12-20
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| 引用本文：胡志慧,姬志勤,魏少鹏.河朔荛花根中抑菌活性成分研究[J].农药学学报,2019,21(2):140-145. |
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| 基金项目:国家重点研发计划（2017YFD0200506）；国家自然科学基金资助项目（31371973，31301700）；陕西省教育厅重点实验室科研计划项目（访学项目，13JS008） |
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| 中文摘要:在活性追踪指导下，采用多种分离技术从河朔荛花根甲醇提取物中分离得到12个化合物，经核磁共振波谱、质谱等技术并结合相关文献确定其结构分别为1-己酸-2，3-硬脂酸甘油三酯（1）、β-谷甾醇（2）、月桂醇（3）、顺-十八碳-9-烯酸（4）、（2E）-庚基-3-（3，4-二羟基苯基）丙烯酸酯（5）、（-）-pluviatolide （6）、1β-hydroxy-10β-H-guaia-4，11-dien-3-one （7）、异狼毒素（8）、新狼毒素B （9）、瑞香酚（10）、单棕榈酸甘油酯（11）和（+）-去甲络石甙元（12）。其中，化合物3、4和6~12均为首次从河朔荛花中分离得到。抑菌活性测定结果表明：异狼毒素（8）和新狼毒素B （9）对马铃薯晚疫病菌Phytophthora infestans菌丝生长和孢子萌发均表现出明显的抑制作用，其中对菌丝生长的EC50值分别为68.4 μg/mL和44.9 μg/mL，对孢子萌发的EC50值分别为25.1 μg/mL和14.0 μg/mL。 |
| 中文关键词:河朔荛花 分离鉴定 抑菌活性 马铃薯晚疫病菌 |
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| Studies on antifungal components in the roots of Wikstroemia chamaedaphne Meisn. |
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| Abstract:In this study, 12 compounds were isolated from the methanol extract of Wikstroemia chamedaphne Meisn. using a variety of separation techniques. Based on the NMR, MS and the data in literatures, they were identified as 1-hexanoic acid-2,3-stearic acid triglyceride (1), β-sitosterol (2), dodecanol (3), cis-octadeca-9-enoic acid(4), (2E)-heptyl-3-(3,4-dihydroxyphenyl) acrylate (5), (-)-pluviatolide (6), 1β-hydroxy-10β-H-guaia-4,11-dien-3-one (7), isochamaejasmin (8), neochamaejasmin B (9), stelleranol (10), glyceryl monopalmitate (11) and (+)-nortrachelogenin (12). Among them, compounds 3, 4 and 6-12 were isolated from this plant for the first time. The results of antifungal activity evaluation indicated that isochamaejasmin (8) and neochamaejasmin B (9) showed significant inhibitory effects on the mycelial growth and the spore germination of Phytophthora infestans. The EC50 values of 8 and 9 were 68.4 μg/mL and 44.9 μg/mL for the growth of P. infestans, respectively. And the EC50 values of 8 and 9 were 25.1 μg/mL and 14.0 μg/mL, respectively, for the spore germination of P. infestans. |
| Key words:Wikstroemia chamedaphne separation and identification Phytophthora infestans antifungal activity |
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