| 竹红菌甲素对番茄灰霉病菌的关键抑菌因子 |
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投稿时间：2018-06-19
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| 引用本文：樊炎迪,赵学,吴酬飞,等.竹红菌甲素对番茄灰霉病菌的关键抑菌因子[J].农药学学报,2019,21(1):59-65. |
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| 基金项目:国家重点研发计划项目子课题（2017YFD0201302-04）；浙江省2011协同创新中心研究基金项目（S20150004-2） |
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| 中文摘要:为探究竹黄菌Shiraia bambuscola代谢产物竹红菌甲素（HA）对番茄灰霉病菌Botrytis cinerea的关键抑菌因子，依据活性氧淬灭原理，测定了HA的光反应类型；采用单线态氧（1O2）捕捉法、芬顿试剂法和核黄素光化学反应法，分析了单线态氧（1O2）、羟基自由基（·OH）和超氧阴离子自由基（O2·）3种活性氧浓度与HA对番茄灰霉病菌抑制率的相关性。结果表明：HA是通过光动力作用中的2种光反应类型（Type I和Type Ⅱ）产生的活性氧对番茄灰霉病菌起到抑制作用，其中主要以Type Ⅱ为主。1O2、·OH和 O2· 的浓度与其对番茄灰霉病菌的抑制率均呈极显著的线性正相关，3种活性氧均为关键抑菌因子。经回归分析，获得最优回归模型为：Y = 0.055 9 + 0.814 5X1 + 0.002X2 + 0.738 4X3，其中Y为抑制率，X1、X2和X3分别为 1O2、·OH和 O2· 的浓度，R2 = 0.961 9，可见三者抑菌能力顺序为 1O2 > O2· > ·OH。本论文首次提出HA抑制番茄灰霉病菌的关键因子，为HA作用机理的研究奠定了基础，并为其作为光活化农药的开发利用提供了理论依据。 |
| 中文关键词:竹红菌甲素 番茄灰霉病菌 关键抑菌因子 抑菌作用 活性氧 光反应类型 |
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| Key factors in the antifungal activity of Hypocrellin A against Botrytis cinerea |
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| Abstract:The key factors in the antifungal activity of Hypocrellin A (HA), the metabolites of Shiraia bambuscola, against the Botrytis cinerea were investigated. The type of photoactivity of HA was determined based on the principle of active oxygen quenching. The correlation between the concentration of three reactive oxygen species, including singlet oxygen (1O2), hydroxyl radical (·OH), and the superoxide anion radical (O2·), and the inhibition rate of HA against B. cinerea was analyzed by the singlet oxygen capture principle, Fenton reaction, and riboflavin photochemical reaction. Results showed that the antifungal activity of HA against B. cinerea resulted from the active oxygen generated by photodynamic action (Type I and Type Ⅱ). Both types exhibited controlling effects against the growth of B. cinerea, but Type Ⅱ exhibited is more active. The concentration of 1O2, ·OH, and O2· are positively correlated with the bacteriostatic rates, and those three active oxygen species were the key factors in the antifungal activity. Stepwise regression analysis revealed that 1O2 is the most critical antifungal factor produced by HA, followed by O2· and ·OH. The optimal regression model was obtained as follows: Y = 0.055 9 + 0.814 5X1 + 0.002X2 + 0.738 4X3, in which Y was the inhibition rate, X1, X2 and X3 were the concentration of 1O2, ·OH and O2·, respectively, R2 = 0.961 9. In this paper, the key factors in the antifungal activity of HA against B. cinerea were investigated for the first time, which laid a foundation for the study of the mechanism of HA and provided a theoretical basis for the development and utilization of HA as a photoactivated pesticide. |
| Key words:Hypocrellin A Botrytis cinerea key inhibition factors inhibition reactive oxygen specie photo reaction type |
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