| 山东省玉米纹枯病菌对噻呋酰胺的敏感性及其抗性突变体的生物学性状 |
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投稿时间：2018-08-21
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| 引用本文：尚佃龙,姜兴印,刘玉,等.山东省玉米纹枯病菌对噻呋酰胺的敏感性及其抗性突变体的生物学性状[J].农药学学报,2018,20(6):735-742. |
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| 基金项目:国家重点研发计划（2018YFD0200604）；山东省现代农业产业技术体系项目（SDAIT-02-10）；山东“双一流”奖补项目（SYL2017-XTTD11） |
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| 中文摘要:为明确山东省玉米纹枯病菌对噻呋酰胺的敏感性及其抗药性风险，采用菌丝生长速率法分别测定了采自山东泰安、临沂、潍坊、莱芜、日照及青岛6个地区的102株玉米纹枯病菌对噻呋酰胺的敏感性。结果表明：其EC50值分布范围为0.010～0.194 μg/mL，平均EC50 值为（0.086 ±0.004）μg/mL，且敏感性呈连续单峰曲线分布。通过紫外诱导与药剂驯化的方法各获得5株耐药性菌株（TA3-X2、TA17-X6、LY8-3、QD14-Y7和WF6-A2）和1株抗性突变体（QD2-Y4），其抗性水平在6.46～20.08倍之间，突变频率分别为0.87%和0.52%。对抗性突变体生物学性状的研究表明，紫外诱导获得的5株耐药性菌株其耐药性不能稳定遗传，而经药剂驯化获得的1株抗性突变体QD2-Y4的抗药性可稳定遗传；耐药性菌株TA3-X2的菌丝生长速率高于亲本菌株，其余菌株与亲本菌株差异不明显；5株耐药性菌株和1株抗性突变体的菌丝干重和菌核干重均低于亲本菌株；TA3-X2、WF6-A2及QD2-Y4的致病力低于亲本菌株，TA17-X6、LY8-3及QD14-Y7的致病力与亲本菌株无明显差异。交互抗性测定表明，噻呋酰胺抗性突变体与戊唑醇、丙环唑、咯菌腈、井冈霉素、苯醚甲环唑和多菌灵之间均无交互抗性，与啶酰菌胺和氟唑菌苯胺之间则存在交互抗性。研究表明，山东省6个地区的玉米纹枯病菌对噻呋酰胺比较敏感，推测噻呋酰胺可作为防治玉米纹枯病的理想候选药剂。 |
| 中文关键词:玉米纹枯病菌 噻呋酰胺 啶酰菌胺 敏感性 诱导抗性 生物学性状 交互抗性 |
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| Sensitivity of Rhizoctonia solani to thifluzamide in Shandong Province and the biological characteristics of resistant mutants |
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| Abstract:In order to clarify the sensitivity and resistance risk of Rhizoctonia solani to thifluzamide in Shandong Province, 102 isolates of R. solani in 6 areas in Shandong, including Tai’an, Linyi, Weifang, Laiwu, Rizhao and Qingdao, were investigated using the mycelial growth rate method. The EC50 values of thifluzamide against different isolates of the pathogen ranged from 0.010 to 0.194 μg/mL, with an average EC50 value of (0.086 ±0.004) μg/mL, which showed a single-peak curve distribution. Five drug-resistant isolates were obtained by UV induction and one resistant mutant was obtained by drug domestication. Their resistance levels ranged from 6.46 to 20.08. The mutation frequencies were 0.87% and 0.52%, respectively. Five drug-resistant isolates obtained by UV induction could not be stably inherited. While one mutant QD2-Y4 obtained by acclimation of the drug could be stably inherited. Studies on the biological traits of antagonistic mutants showed that the mycelial growth rate of TA3-X2 isolate was higher than that of the parent isolates, and no other obvious difference was detected. The dry weight of mycelium and the dry weight of sclerotia of the five resistant isolates and one resistant mutant were all lower than the parent isolates. The pathogenicity of TA3-X2, WF6-A2 and QD2-Y4 was lower than that of the parent isolates. However, the pathogenicity of TA17-X6, LY8-3 and QD14-Y7 was not significantly different from that of the parental isolates. There was no cross-resistance between thifluzamide and some other fungicides, including tebuconazole, propiconazole, fludioxonil, validamycin, difenoconazole and carbendazim. However, the cross-resistance among thifluzamide, boscalid and penflufen was observed. Overall, R. solani is sensitive to thiafuramide in 6 areas of Shandong Province. It is speculated that thiafidamide can be used as an ideal candidate for controlling banded leaf sheath blight in corn. |
| Key words:Rhizoctonia solani thifluzamide boscalid sensitivity resistance induction biological characteristics cross resistance |
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