浙江省果蔬灰葡萄孢对啶酰菌胺的抗性
投稿时间：2018-08-25      点此下载全文 HTML
引用本文：林泽松,赵帅锋,皇甫运红,等.浙江省果蔬灰葡萄孢对啶酰菌胺的抗性[J].农药学学报,2018,20(6):729-734.
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作者单位E-mail
林泽松 浙江农林大学 农业与食品科学学院, 杭州 311300  
赵帅锋 浙江省建德市植物保护站, 浙江 建德 311600  
皇甫运红 浙江农林大学 农业与食品科学学院, 杭州 311300  
吴鉴艳 浙江农林大学 农业与食品科学学院, 杭州 311300  
王华弟 浙江省农药检定管理总站, 杭州 310004  
张传清 浙江农林大学 农业与食品科学学院, 杭州 311300 cqzhang@zafu.edu.cn 
基金项目:浙江省重点研发项目（2015C02G1320008）
中文摘要:以2004—2006年从浙江、江苏等地采集的灰葡萄孢对啶酰菌胺的敏感性基线[EC50 =（1.07 ±0.11）mg/L]为依据，采用菌丝生长速率法连续监测了浙江省果蔬灰葡萄孢群体对啶酰菌胺的敏感性变化。结果表明：浙江省果蔬灰葡萄孢对啶酰菌胺的抗性发展迅速，2012—2013年和2017—2018年的平均EC50值分别为（5.23 ±7.79）和（24.30 ±49.33）mg/L。其中，2012—2013年的抗药性菌株频率为15.3%，且均为低水平抗性（LR）菌株；而2017—2018年的抗药性频率上升至53.2%，并出现了7.5%的中等水平抗性（MR）菌株和1.3%的高水平抗性（HR）菌株。啶酰菌胺抗性菌株的菌丝生长速率、产孢量、产菌核数和致病力与敏感菌株相比均无显著差异。抗药性分子机制研究表明：啶酰菌胺抗性菌株的琥珀酸脱氢酶B亚基（SDH B）均发生了点突变，共包括H272R、P225F和N230I 3种类型，其中H272R型突变占88.5%；其SDH A和SDH D均未发生点突变；而SDH C的突变（G85A + I93V + M158V + V168I）与对药剂敏感性之间无明显联系。
中文关键词:灰葡萄孢  琥珀酸脱氢酶抑制剂  啶酰菌胺  抗药性监测  抗药性机制
 
Resistance to boscalid in Botrytis cinerea in Zhejiang Province
Abstract:Shift of sensitivity to boscalid in Botrytis cinerea collected from plastic tunnels growing fruits and vegetables in Zhejiang Province was monitored by the mycelial growth rate assay based on our previously reported sensitivity baseline, (1.07 ±0.11) mg/L for the wild sensitive isolates collected from 2004 to 2006. The results showed that resistance to boscalid in B. cinerea was developed rapidly. The mean EC50 was (5.23 ±7.79) and (24.30 ±49.33) mg/L, respectively for isolates collected during 2012 to 2013 and during 2017 to 2018. The total resistance frequency during 2012 to 2013 was 15.3% and all resistant isolates were low-level resistant (LR). For the isolates collected from 2017 to 2018, the total resistance frequency was increased to 53.2%, including 7.5% MR isolates and 1.3% HR isolates. There was no significant difference in mycelial growth, sporulation, sclerotial production, and pathogenicity between boscalid resistant and sensitive isolates. No point mutation was detected in SDH A and SDH D. However, a single point mutation, H272R, P225, or N230I, was found in SDH B of all boscalid-resistant B. cinerea. Among them, 88.5% of resistant isolates were H272R. And no obvious association was found between boscalid-resistance and mutations (G85A + I93V + M158V + V168I) in SDH C.
Key words:Botrytis cinerea  succinate dehydrogenase inhibitor (SDHIs)  boscalid  resistance monitoring  resistance mechanism
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