| 三甲基硅重氮甲烷衍生化-气相色谱法测定水果蔬菜中乙烯利的残留 |
|
投稿时间：2018-04-14
点此下载全文
HTML
|
| 引用本文：陈克云,王艳丽,沈祥震,等.三甲基硅重氮甲烷衍生化-气相色谱法测定水果蔬菜中乙烯利的残留[J].农药学学报,2018,20(4):495-499. |
| 摘要点击次数: 1695 |
| 全文下载次数: 589 |
|
|
| 中文摘要:针对水果蔬菜中乙烯利的残留，建立了三甲基硅重氮甲烷衍生化-气相色谱的分析方法。样品用V（甲醇）：V（盐酸）=9：1的溶液提取，无水乙醚萃取，三甲基硅重氮甲烷衍生化15 min，采用气相色谱-质谱法定性，气相色谱仪-火焰光度检测器（GC-FPD）检测，外标法定量。结果显示：在0.01~2 mg/L范围内，乙烯利的质量浓度与其对应的峰面积间线性关系良好，相关系数大于0.999 6；此方法检出限为0.01 mg/kg，定量限为0.03 mg/kg；在辣椒、番茄、香蕉、苹果和梨的空白果蔬样品中分别添加0.01、0.1和1 mg/kg 3个水平下，回收率为93%~102%，相对标准偏差为4.2%~7.3%。该方法准确度和灵敏度高，抗干扰能力强，能准确并快速测定出水果、蔬菜中乙烯利的残留量。 |
| 中文关键词:三甲基硅重氮甲烷 衍生化 气相色谱 水果 蔬菜 乙烯利 残留 |
| |
| Determination of ethephon residue in fruits and vegetables by gas chromatography after derivatization with (trimethylsilyl)diazomethane |
|
|
| Abstract:An analytical method for the determination of ethephon residue in fruits and vegetables was established by using gas chromatography after derivatization with (trimethylsilyl)diazomethane. The samples were extracted with a mixture of hydrochloric acid and methanol (9:1, V/V). It was then extracted with anhydrous ether and subjected to derivatization by (trimethylsilyl)diazomethane. The extracts were determined by gas chromatography with the external standard method and qualitative by gas chromatography-mass spectrometry. The calibration curve of ethephon residue was linear in the range of 0.01-2 mg/L with correlation coefficients higher than 0.999 6. The detection limit of this method was 0.01 mg/kg and the limit of quantification was 0.03 mg/kg. In the samples of fruits and vegetables at spiked levels from 0.01 mg/kg to 1mg/kg, the recovery was 93%-102% with the relative standard deviation from 4.2%-7.3%. The method has the advantages of high accuracy, good sensitivity, and strong anti-interference ability, which can be used for the accurate and rapid determination of ethephon residues in fruits and vegetables. |
| Key words:(trimethylsilyl)diazomethane derivatization gas chromatography fruit vegetable ethephon residue |
|
查看全文
查看/发表评论
下载PDF阅读器 |
