胶孢炭疽菌生防放线菌gz-8的鉴定及生物活性初步评价 |
投稿时间:2017-06-27
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引用本文:张凯,吴曼莉,辜柳霜,等.胶孢炭疽菌生防放线菌gz-8的鉴定及生物活性初步评价[J].农药学学报,2017,19(6):708-715. |
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基金项目:海南省重点研发计划(ZDYF2016155);国家自然科学基金(31560045) |
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中文摘要:从山东省临沂地区土壤中分离到一株放线菌gz-8,发现其对胶孢炭疽菌Colletotrichum gloeosporioides具有较强的抑制作用,皿内抑菌活性达72.5%。通过形态特征和生理生化测定,结合16S rDNA序列分析,鉴定该菌株为栗褐链霉菌Streptomyces badius。抗菌谱测定结果表明,菌株gz-8对6种植物病原真菌均具有较强的抑菌活性,抗菌谱较广。进一步对菌株gz-8的粗提物进行了生物活性评价,发现其发酵液粗提物可以明显抑制胶孢炭疽菌的菌丝生长(EC50=3.18 μg/mL)和分生孢子萌发(IC50=1.92 μg/mL),且活性明显高于菌体粗提物;同时发酵液粗提物具有良好的热稳定性及pH稳定性;1 000 mg/L的发酵液粗提物对感染胶孢炭疽病芒果果实的防效达到35.94%,与同浓度下百菌清的防效(34.11%)无显著差异。 |
中文关键词:胶孢炭疽菌 放线菌 栗褐链霉菌 炭疽病 菌株鉴定 生物防治 |
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Identification and preliminary evaluation of a biocontrol actinomycete strain gz-8 against Colletotrichum gloeosporioides |
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Abstract:An actinomycete strain gz-8 was isolated from Linyi, Shandong Province, which had a strong antagonistic effect against Colletotrichum gloeosporioides with the inhibition rate of 72.5%. Strain gz-8 was identified as Streptomyces badius based on the morphology, physiological and biochemical characteristics, combined with 16S rDNA sequence analysis. Strain gz-8 has a broad antifungal spectrum, with strong inhibitory effects on six plant pathogenic fungi. The inhibitory activity of the crude extract from strain gz-8 was evaluated. The fermentation broth extract exhibits obvious inhibitory effects on the mycelial growth (EC50=3.18 μg/mL) and conidial germination (IC50=1.92 μg/mL) of C. gloeosporioides, which had higher activity compared to the mycelium extract of strain gz-8. The fermentation broth extract had good thermostability and pH stability. The control effect of 1 000 mg/L fermentation broth extract to mango fruits infected with C. gloeosporioides was 35.94%, which had no significant difference with that of chlorothalonil at the same concentration(34.11%). |
Key words:Colletotrichum gloeosporioides anthracnose actinomycete strain Streptomyces badius strain identification biological control |
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