氯苯醚酰胺(Y13149)生物活性初步研究及水稻纹枯病菌对其敏感性基线的建立
投稿时间：2017-06-21      点此下载全文 HTML
引用本文：戴德江,魏方林,王琼伟,等.氯苯醚酰胺(Y13149)生物活性初步研究及水稻纹枯病菌对其敏感性基线的建立[J].农药学学报,2017,19(5):550-554.
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作者单位E-mail
戴德江 浙江农林大学 农业与食品科学学院, 杭州 311300
浙江省农药检定管理总站, 杭州 310004 		 
魏方林 江西省农药工程技术研究中心, 南昌 330096  
王琼伟 浙江农林大学 农业与食品科学学院, 杭州 311300  
吴鉴艳 浙江农林大学 农业与食品科学学院, 杭州 311300  
张传清 浙江农林大学 农业与食品科学学院, 杭州 311300 cqzhang@zafu.edu.cn 
基金项目:浙江省重点研发项目（2015C02G2010084）；浙江公益技术应用研究计划（2016C32002）；浙江省“三农六方”项目
中文摘要:氯苯醚酰胺[N-(2-(2,4-二氯苯氧基)苯基)-3-(二氟甲基)-1-甲基-1H-吡唑-4-酰胺，Y13149]是由华中师范大学基于药效团连接碎片虚拟筛选策略(PFVS)快速发现的新型琥珀酸脱氢酶抑制剂类(SDHIs)化合物。采用菌丝生长速率法，测定了该创制化合物的农药生物活性。结果表明：氯苯醚酰胺对真菌具有较广的抗菌谱，对供试10种植物病原真菌均具有较好的抑制活性，EC50值在0.008~15.25 mg/L之间，其对立枯丝核菌的抑制活性最强，但对辣椒疫霉和链状腐霉活性较弱。田间试验表明，氯苯醚酰胺对水稻纹枯病具有较好的防治效果，有效剂量75 g/hm2时的平均防效为79.6%，其防效总体略好于噻呋酰胺(75 g/hm2的平均防效为77.3%)。此外，氯苯醚酰胺对采自浙江和江苏两省的125株水稻纹枯病菌的EC50值分布在0.002~0.259 mg/L之间，且呈近似正态单峰曲线，平均EC50值为(0.021 ±0.008) mg/L，因此可将其作为水稻纹枯病菌对氯苯醚酰胺的敏感性基线，用于田间抗药性监测。
中文关键词:氯苯醚酰胺  琥珀酸脱氢酶抑制剂  生物活性  立枯丝核菌  水稻纹枯病  敏感性基线
 
Bioactivity of Y13149 and its sensitivity baseline of Rhizoctonia solani
Abstract:The bioactivity of Y13149, N-(2-(2,4-dichlorophenoxy) phenyl)-3-(difluoromethyl)-1-methyl-1H-pyrazole-4-carboxamide, a novel succinate dehydrogenase inhibitor discovered through pharmacophore-linked fragment virtual screening by Huazhong Normal University, was evaluated by the method of mycelial growth rate. Results indicated that it exhibited good inhibitory activities against 10 tested plant-pathogenic fungi with the EC50 of 0.008-15.25 mg/L and the highest inhibition rate was observed in the case of Rhizoctonia solani. However, Y13149 had lower activities against Phytophthora capsici and Pythium catenulatum. In field experiments, the control efficacy of Y13149 for the rice sheath blight disease is, on average, 79.6% at the dose of 75 g/hm2, which is comparable with that of trifuzamide (77.3% at the dose of 75 g/hm2). Further study suggested that the EC50 for the tested population (n=125) of R.solani collected from Zhejiang and Jiangsu Province fitted unimodal distribution and varied from 0.002 to 0.259 mg/L with the mean of (0.021 ±0.008) mg/L, which could be adopted as the baseline sensitivity of R.solani to Y13149 to monitor the sensitivity shift.
Key words:Y13149  succinate dehydrogenase inhibitor (SDHIs)  bioactivity  Rhizoctonia solani  rice sheath blight  sensitivity baseline
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