Cylindrocarpon olidum W1菌株代谢物中壳二孢氯素及其类似物的分离及抑菌活性
投稿时间:2017-06-09      点此下载全文 HTML
引用本文:魏少鹏,姬志勤.Cylindrocarpon olidum W1菌株代谢物中壳二孢氯素及其类似物的分离及抑菌活性[J].农药学学报,2017,19(4):457-464.
摘要点击次数: 105
全文下载次数: 28
作者单位E-mail
魏少鹏 西北农林科技大学 植物保护学院, 陕西 杨凌 712100;西北农林科技大学 陕西省植物源农药研究与开发重点实验室, 陕西 杨凌 712100  
姬志勤 西北农林科技大学 植物保护学院, 陕西 杨凌 712100;西北农林科技大学 陕西省植物源农药研究与开发重点实验室, 陕西 杨凌 712100 jizhiqin@nwsuaf.edu.cn 
基金项目:国家重点研发计划(2017YFD0200506);国家自然科学基金资助项目(31371973,31301700);陕西省科技攻关项目(2016NY-139)
中文摘要:采用硅胶柱层析及高效液相色谱等技术从冬青卫矛内生真菌Cylindrocarpon olidum W1次生代谢物中分离鉴定出8个抑菌活性化合物W1-1~W1-8。经核磁共振氢谱和碳谱、高分辨质谱等技术,并结合相关文献确认其为壳二孢氯素及其类似物,分别为Ilicicolin A(W1-1)、5-Chlorocolletorin B(W1-2)、Ilicicolin B(W1-3)、Deacetylchloronectrin(W1-4)、Ilicicolin C(W1-5)、壳二孢氯素(W1-6)、Cylindrol A4W1-7)和Cylindrol B(W1-8)。抑菌活性测定结果表明:壳二孢氯素及其类似物对烟草青枯病菌有较强的抑制作用,上述化合物的最小抑制浓度分别为6.25、3.13、>;100、3.13、6.25、12.5、25和25 μg/mL;化合物W1-2W1-4W1-5对番茄灰霉病菌和油菜菌核病菌菌丝生长亦有较强的抑制作用,其中对番茄灰霉病菌的抑制中浓度(IC50)分别为36.45、21.60和26.69 μg/mL,对油菜菌核病菌的抑制中浓度分别为20.21、16.79和12.11 μg/mL。
中文关键词:Cylindrocarpon olidum W1  壳二孢氯素  分离鉴定  抑菌活性
 
Isolation and antimicrobial activities of ascochlorin and its analogues from the metabolites of Cylindrocarpon olidum W1
Abstract:Eight compounds W1-1–W1-8 were isolated from the secondary metabolites of Cylindrocarpon olidum W1, which is an endophytic fungus from Euonymus japonica, by silica gel column chromatography and pre-HPLC techniques. Based on the 1H NMR and 13C NMR and HRMS-MS data, as well as the published literatures, these chemicals were identified as Ilicicolin A (W1-1), 5-Chlorocolletorin B (W1-2), Ilicicolin B (W1-3), Deacetylchloronectrin (W1-4), Ilicicolin C (W1-5), Ascochlorin (W1-6), Cylindrol A4 (W1-7) and Cylindrol B (W1-8). The obtained Ascochlorin and its analogues showed strong antibacterial activities against Ralstonia solanacearum, with minimum inhibitory concentrations (MICs) of 6.25, 3.13, > 100, 3.13, 6.25, 12.5, 25 and 25 μg/mL, respectively. Compounds W1-2, W1-4 and W1-5 could also effectively inhibit the mycelial growth of plant pathogenic fungi Botrytis cinerea and Sclerotinia sclerotiorum. Their medium inhibitory concentrations (IC50) against B. cinerea were 36.45, 21.60 and 26.69 μg/mL, respectively. Their IC50 values against S. sclerotiorum were 20.21, 16.79 and 12.11 μg/mL, respectively.
Key words:Cylindrocarpon olidum W1  ascochlorin  isolation and identification  antimicrobial activity
查看全文   查看/发表评论   下载PDF阅读器
&et=D596B4B1B0419C6D2044E4BCA28907FC7B63DB7A59F5EBFDA8F0BC8D1E1F77F6C3F0BB59CA3B4E06E88F05A14FCFF7F5061D40430ECB477A0EEB323DD2A13A1FF64531D33B2EED06333A4A352AD8E376C28824ACAE98CFF4F45B769792236977E611CEA1B8EAB540C5AEE46BED0093716AA8CB180DC0AD0D2AA279B13602CBE4&pcid=03F54A49DE00578AA0E5DDF5BC021AA7&cid=B6C161C332FA44D9&jid=C858D086464CADF4E2006AC1A8358566&yid=FA004A8A4ED1540B&aid=3C3EF3B75913B4FFFB41ADCEFC92E8EE&vid=&iid=E158A972A605785F&sid=D0E8F9CBDBE0070C&eid=1A033C02510EFBE6&fileno=20170407&flag=1&is_more=0"> var my_pcid="03F54A49DE00578AA0E5DDF5BC021AA7"; var my_cid="B6C161C332FA44D9"; var my_jid="C858D086464CADF4E2006AC1A8358566"; var my_yid="FA004A8A4ED1540B"; var my_aid="3C3EF3B75913B4FFFB41ADCEFC92E8EE";