纳米标记免疫层析法在农药残留检测中的应用研究进展
投稿时间:2017-03-22      点此下载全文 HTML
引用本文:司芳芳,郭逸蓉,赵颖,桂文君,王蒙岑,朱国念,等.纳米标记免疫层析法在农药残留检测中的应用研究进展[J].农药学学报,2017,19(4):409-417.
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作者单位E-mail
司芳芳 浙江大学 农药与环境毒理研究所, 杭州 310058  
郭逸蓉 浙江大学 农药与环境毒理研究所, 杭州 310058 yirongguo@zju.edu.cn 
赵颖 浙江大学 农药与环境毒理研究所, 杭州 310058  
桂文君 浙江大学 农药与环境毒理研究所, 杭州 310058  
王蒙岑 浙江大学 农药与环境毒理研究所, 杭州 310058  
朱国念 浙江大学 农药与环境毒理研究所, 杭州 310058  
基金项目:国家自然科学基金(31401768);浙江省公益技术应用研究项目(2016C32004);国家重点研发计划项目(2016YFD0201302)
中文摘要:农药残留超标已成为影响农产品质量安全的重要问题,迫切需要探寻开发灵敏、准确、可靠、便捷且适用性强的农药残留快速检测方法。免疫层析法是将抗原抗体特异性免疫反应和色谱层析分离技术相结合的一种快速检测方法,其中,基于胶体金标记的免疫层析技术以其便捷、成本低、可视化等优点而受到普遍欢迎。近年来随着量子点、时间分辨荧光微球、上转换发光纳米粒子等新型纳米标记材料的出现,免疫层析技术得到了广泛发展。文章从标记类型(非共价作用标记及共价作用标记)及标记材料(胶体金、纳米碳、量子点、上转换发光纳米粒子、磁性纳米颗粒、时间分辨荧光微球及荧光乳胶颗粒)等方面,综述了不同纳米材料标记的免疫层析技术及其在农药残留检测领域的研究及应用进展,可为深入开展农药残留免疫层析技术研究提供参考。
中文关键词:农药残留  免疫层析技术  纳米标记材料  胶体金  量子点  上转换发光纳米粒子
 
Research advances of nanomaterial-labelled immunochromatography and application in pesticide residue determination
Abstract:The excessive pesticide residues have become important issues influencing the quality and safety of agricultural products. Thus, it is urgent to develop a sensitive, accurate, reliable, convenient and widely applicable method for the determination of pesticide residues. Immunochromatographic assay (ICA) is a type of rapid detection technology that combines the specific immune response of antigen and antibody and chromatography separation technology. ICA technology based on colloidal gold is well accepted for its convenience, low cost and visualization. In recent years, with the development of nanometer materials such as quantum dots (QDs), time-resolved fluorescence microspheres and upconversion nanoparticles, ICA has been extensively developed. The ICAs labeled by different nanoparticles and their recent application in pesticide residues detection were reviewed in label types (non-covalent and covalent labeling) and label materials (colloidal gold, nanocarbon, quantum dots, upconversion nanoparticles, magnetic nanoparticles, time-resolved fluorescence microspheres and latex microspheres). This review will helpful for future research in the field of immunochromatography for pesticide residue.
Key words:pesticide residues  immunochromatographic assay  nanometer materials  colloidal gold  quantum dots  upconversion nanoparticles
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