| 杠柳杀虫活性成分的分离 |
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投稿时间：2011-07-26
修订日期：2011-10-23
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| 引用本文：师宝君,高履桐,姬志勤,等.杠柳杀虫活性成分的分离[J].农药学学报,2012,14(1):103-106. |
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| 基金项目:国家重点基础研究发展计划(973)项目 (2010CB126105); 国家公益性行业(农业)科研专项(200903052); 国家自然科学基金 (30870344,31071704);西北农林科技大学青年学术骨干项目. |
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| 中文摘要:采用柱层析分离、高效液相色谱切分和生物活性追踪法,从杠柳 Periploca sepium 根皮甲醇提取物中分离出2个具有杀虫活性的化合物(G1和G2),经鉴定其分别为已知物杠柳新苷D和F。生物活性测定结果表明,化合物G1和G2 对3龄粘虫 Mythimna separata 48 h的胃毒致死中浓度(LC50)分别为0.39和0.34 mg/mL, 对小菜蛾 Plutella xyllostella 48 h的胃毒LC50值分别为1.21和1.39 mg/mL。 |
| 中文关键词:杠柳 杀虫活性 分离 杠柳新苷 |
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| Isolation of the insecticidal ingredients from Periploca sepium |
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| Abstract:Two compounds,periplocoside D and F were isolated from the methanol extract of the root barks of Periploca sepium by silica gel chromatograph,high pressure liquid chromatograph(HPLC) and bioassay-guided fractionation.The bioassay results showed that periplocoside D and F had stomach toxic activity with the LC50 value of 48 h 0.39 and 0.34 mg/mL against 3rd instar larvae of Mythimna separata,and against 3rd instar larvae of Plutella xyllostella with the LC50 value of 48 h 1.21 and 1.39 mg/mL. |
| Key words:Periploca sepium insecticidal activity isolated periplocoside |
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